SPEAKERS: Yakisich, J. Sebastian, MD, PhD

Assistant Professor

Department of Clinical Neuroscience

Karolinska University Hospital

Karolinska Institute

Stockholm, Sweden                                                                             

Dr Yakisich received his M.D. from University of Buenos Aires (1994) and his Ph.D. in Neurology from the Karolinska Institute (2005). He received postdoctoral training at Dr. Kapler's lab in the Health Science Center (Texas A&M University, TX, USA).

He was appointed Assistant Professor at the Department of Clinical Neuroscience,  Karolinska Institute (Stockholm, Sweden) in 2008.

He also serves as reviewers of several scientific journal and is Editor-In-Chief of Drugs & Therapy Studies.

Dr. Yakisich's group has developed new concepts and strategies for cancer treatment:

Regrowth concentration zero (RC₀)

Pankiller drugs, Read the article (PDF)

Stemness Phenotype Model  of glioma biology. Read the article (PDF)

Two phases treatment (2FT) to eliminate drug-surviving cells as strategy to prevent tumor relapse. Read the article (PDF)

Publications

27. Ferraro, S.A., Gallo, F.T., Yakisich J.S. and Tasat D.R. (2011) Simvastatin pretreatment prevents ambient particle-induced lung injury in mice. Inhalation Toxicol. In Press.

26. Delwar, Z., Avramidis D., Siden, Å., Cruz, M., Yakisich, J.S.  (2011) Depletion of drug-surviving glioma cells by a second phase treatment with low concentration of salinomycin. Drugs and Therapy Studies. 1:e7 Abstract

25. Delwar, Z., Vita, M.F. Siden, Å., Cruz, M. and Yakisich, J.S. (2011) In vitro inhibition of topoisomerase II by reduced glutathione. Acta Biochim Polonica. 58: 265-267 PubMed

24. Delwar, Z., Avramidis, D., Follin, E., Hua, Y., Siden Å., Cruz, M., Paulsson, K.M. , Yakisich, J.S. (2011) Cytotoxic effects of menadione:sodium orthovanadate combination on human glioma cells. Invest New Drugs PubMed

23. Vita, M.F, Nagachar, N., Avramidis,  D., Delwar, Z., Cruz, M., Siden Å., Paulsson, K.M., Yakisich, J.S. (2011) Pankiller effect of prolonged exposure to menadione on glioma cells: potentiation by vitamin C. Invest New Drugs. 29: 1314-1320. PubMed. 

22. Martin S., Fernandez-Alanis, E., Delfosse, V., Evelson, P., Yakisich, J.S., Saldiva, P.H., Tasat D.R. (2010) Low doses of urban air particles from Buenos Aires promote oxidative stress and apoptosis in mice lungs. Inhalation Toxicol. 22:1064-71 PubMed.

21. Cruz M., Siden Å., Tasat D.R., Yakisich, J.S. (2010) Are all Glioma Cells Cancer Stem Cells? J Cancer Sci Ther 2: 100-106 PDF  

20. Yakisich, J.S., Vita, M., Tasat, D.R., Sidén, Å., and  Cruz, M. (2010) Strong inhibition of replicative DNA synthesis in the developing rat cerebral cortex and glioma cells by roscovitine. Invest New Drugs. 28:299-305. PubMed

19. Yakisich, J.S., Sidén, Å., and  Cruz, M. (2010) Ongoing DNA synthesis in the rat cerebral cortex is regulated by a proteolytic pathway independent of the proteasome and calpains. Invest New Drugs. 28:242-250. PubMed 

18. Avramidis, D., Cruz, M., Sidén, Å., Tasat, D.R., Yakisich, J.S. (2009)  Regrowth Concentration Zero (RC0) as complementary endpoint parameter to evaluate compound candidates during preclinical drug development for cancer treatment. J. Cancer Sci. & Ther. 1, 19-24. PDF

17. Yakisich, J.S., (2009) Theoretical basis for the measurement of small differences in the length of the cell cycle between two cell populations. The Open Biology Journal. 2:95-100.

16. Yakisich, J.S., Ohlsson Lindblom, I., Siden, Å., and Cruz, M.H. (2009) Rapid inhibition of ongoing DNA synthesis in human glioma tissue by genistein  Oncol. Rep. 22: 569-574. PubMed

15. Ostachuk, A., Evelson, P., Martin, S., Dawidowski, L., Yakisich, J.S. and Tasat, D.R. (2008) Age-related lung cell response to Urban Buenos Aires Air Particle soluble fraction. Environmen. Res. 107:170-7. PubMed

14. Mohammad, M.M., Donti, T.R., Yakisich, J.S., Smith, A.G. and G.M. Kapler. (2007) Tetrahymena ORC contains a ribosomal RNA fragment that participates in rDNA origin recognition. EMBO J. 26:5048-5060  Pubmed   This article was cited in F1000 (http://f1000.com/1097348 ).

13. Yakisich, J.S*, Sandoval, P*., Morrison, T., and G.M. Kapler. (2006) TIF1 activates the intra-S phase checkpoint response in the diploid micronucleus and amitotic polyploid macronucleus of Tetrahymena . Mol. Biol Cell 17:5185-5197. * First co-authors. PubMed

12. Yakisich, J.S. and Kapler, G.M. (2006) Deletion of the Tetrahymena thermophila rDNA replication fork barrier region disrupts macronuclear rDNA excision and creates a fragile site in the micronuclear genome. Nucleic Acid Res. 34:620-634 Pubmed.

11. Morrison, T.L., Yakisich, J.S., Cassidy-Hanley, D. and Kapler, G.M. (2005) TIF1 Represses rDNA Replication Initiation, but Promotes Normal S Phase Progression and Chromosome Transmission in Tetrahymena. Mol. Biol. Cell 16 2624-2635 PubMed 

10. Smith, J.J.*, Yakisich, J.S.* Kapler, G.M., Cole, E.S., and Romero, D.P. (2004) A ß-Tubulin Mutation Selectively Uncouples Nuclear Division and Cytokinesis in Tetrahymena thermophila. Eukaryot. Cell 3: 1217-1226. *First Co-authors. PubMed

9. Yakisich, J.S. and Kapler, G.M. (2004) The effect phosphoinositide 3-kinase inhibitors on programmed nuclear degradation in Tetrahymena thermophila and fate of surviving nuclei . Cell Death Differ. 11: 1146-1149. PubMed

8. Vitali, L.,Yakisich, J.S., Vita, M.F., Fernandez, A., Settembrini, L., Sidén, Å., Cruz, M., Carminatti, H., Casas, O. and Idoyaga Vargas, V. (2002) Roscovitine inhibits ongoing DNA synthesis in human cervical cancer. Cancer Lett. 180: 7-12. PubMed

7. Yakisich, J.S., , Sidén, Å., Eneroth, P., and Cruz, M. (2001) Disulfiram is a potent in vitro inhibitor of DNA topoisomerases. Biochem. Biophys. Res. Commun. 289: 586-590. PubMed

6. Yakisich, J.S., Boethius, J., Walsteed, L., Ohlsson Lindblom, I., Idoyaga Vargas, V., Siden, A., and Cruz, M. (1999) Inhibition of DNA synthesis in human gliomas by roscovitine. Neuroreport 10: 2563-2567. PubMed

5. Yakisich, J.S., Idoyaga Vargas, V., Eneroth, P., Sidén, Å. and Cruz, M. (1999) Early effects of protein kinase modulators on DNA synthesis in rat cerebral cortex. Exp. Neurol. 159: 164-176. PubMed

4. Yakisich, J.S., Siden, A., Idoyaga Vargas, V., Eneroth, Peter and Cruz, M. (1998) Fast and sensitive method for simultaneous measurement of cell proliferation rate and drug sensitivity in rat cerebral cortex. Exp. Neurol. 151:194-202. PubMed

3. Yakisich, J.S., Siden, A., Idoyaga Vargas, V., Eneroth, Peter and Cruz, M. (1998) Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine. Biochem. Biophys. Res. Commun. 243, 674-677. PubMed

2. Castagnino, G., Yakisich, J.S., Iacono, R., Berria, M.I.and Idoyaga Vargas, V. (1995) Growth inhibition of Herpes Simplex Virus Type I in Calphostin-C treated astrocytes. Intervirology 38:332-338. PubMed

1. Yakisich, J.S., Radrizzani, M. and Idoyaga Vargas, V. (1994)Examination of the natural protein substrates affected by Staurosporine in the developing cerebral cortex. Neurosci. Lett. 180:17-20. PubMed

EDITORIALS

 Yakisich, J.S. (2011) Preclinical anticancer drugs screening:  perspectives from emerging models of glioma biology. Drugs & Therapy Studies. 1:e1. Link